Molecular cloning

We offer a wide-range of traditional and modern molecular biology techniques alongside an extensive library of vectors suitable for bacterial, insect, and mammalian systems.

Bacterial expression (E. coli):
 
For expression in E. coli, we use common backbones facilitating inducible expression from T7 promoter and offering a wide range of tags for purification or detection purposes.

Baculovirus expression (BEVS) in insect cells:

For creating expression constructs for the BEVS pipeline, be it for single-gene expression or multi-gene expression from the same construct, we utilize an in-house developed system called GoldenBac (Neuhold et al., 2020).

Transient mammalian expression (HEK 293):

For transient expression in the mammalian system, we usually clone the genes into a backbone with the strong constitutive promoter CAG.

Stable pool mammalian expression (HEK 293):

We utilize a modular vector system equipped with PiggyBac transposition elements and an inducible promoter that also allows for free choice between several antibiotic selection markers.

Gene synthesis:

Should you want to initiate a cloning request, but not have a sequence-verified template for your gene of interest, we can take over the synthesis order and place it with one of the leading vendors on the market.

Custom-cloning:

Upon request, we also take on highly customized cloning projects. The main pre-requisite for such a project is a well-defined goal and verified starting material.

Order forms:

When opening a new ticket to request a service, please download the relevant form for your chosen system:
     - cloning
     - BEVS
     - HEK293

Please complete the form and attach it to your ticket.

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