Molecular cloning

We offer a wide-range of traditional and modern molecular biology techniques alongside an extensive library of vectors suitable for bacterial and mammalian systems.

Bacterial expression system
For expression in E. coli, we use common backbones facilitating inducible expression from T7 promoter and offering a wide range of tags for purification or detection purposes.

Baculovirus-mediated expression in insect cells
For creating expression constructs for BEVS pipeline, be it for single-gene expression or multi-gene expression from the same construct, we utilize the in-house developed system called GoldenBac (Neuhold et al., 2020).

Transient expression in HEK-293 cells
For transient expression in mammalian system, we usually clone the genes into a backbone with strong constitutive promoter CAG.

Stable pool creation in HEK-293 cells
We utilize modular vector system equipped with PiggyBac transposition elements and an inducible promoter that also allows for free choice between several antibiotic selection markers.

Gene synthesis
Should you want to initiate a cloning request, but do not have a sequence-verified template for your gene of interest, we can take over the synthesis order and place it with one of the leading vendors on the market.

Custom-cloning
We are also willing to take on highly customized cloning projects. The main pre-requisite for such a project is well-defined goal and verified starting material.

Order forms
Depending on the chosen service, and while opening a new ticket requesting a service, download the relevant form for:
     - cloning
     - BEVS
     - HEK293
fill in the required information and attach them to the ticket.