Biophysical characterization

We offer protein characterization services upon request using a variety of biophysical techniques to study protein stability, oligomeric state, and secondary structure, or to analyze biomolecular interactions. VBCF ProTech is equipped with:
    - CD, DLS, SEC-MALS, and nDSF for biophysical characterisation.
    - ITC, MST, Monolith X, and GCI for biomolecular interaction. 

To gain a more detailed list of the techniques available, please see the equipment section.

We also provide instrument training and access for some of these methods.  Once access is approved, VBCF ProTech users may book an instrument using our online booking system. Please ensure you confirm staff availability if you will require support.

Our users also have access to a dedicated Data Analysis PC on premises with all software packages related to the instruments we have. This includes:
    - Chirascan and Global3 for the analysis of CD data.
    - Dynamics for the analysis of DLS data.
    - MicroCal PEAQ-ITC analysis software for the analysis of ITC data.
    - MO.Affinity and PALMIST for the analysis of MST data.
    - GraphPad Prism for data plotting and overall analysis.
    - NITPIC, Sedfit, Sedphat, and Sednterp for ITC data analysis and general biophysical queries (i.e. global fitting, extinction coefficients, and dn/dc).

For registered users, more information about data analysis, literature, and online platforms can be found on the resources page, accessible after logging in.