Grating Coupled Interferometry (GCI)

Application: Biomolecular interaction

Instrument: CreoptixWAVE (Malvern Panalytical)

Location: VBC6, room E34

Grating Coupled Interferometry (GCI) a surface-based technology for measuring biomolecular interactions in real-time. The GCI instrument (namely CreoptixWAVE) detects the changes in the refractive index within an evanescent field along the sensor chip. In a GCI experiment, one molecule (the Ligand) is immobilized on a sensor chip and binding of a second molecule (the Analyte) is measured under flow. For any given interactant, the response is a phase shift that is proportional to the mass, and therefore the number of molecules bound to the surface. The increase in surface mass (pg/mm2) is directly monitored as a function of time and is displayed as a sensorgram in real time.

Therefore, GCI experiments can be used to measure and eventually fit kinetic binding constants (ka, kd) and equilibrium dissociation constant KD. Additional experiments conducted over a temperature range would eventually permit the determination of the enthalpy ΔH from a van’t Hoff plot.

Measurements can be performed by the users themselves after having received a compulsory training.
Measurements can also be performed by ProTech staff upon request.